20kHz 1500w Ultrasonic circulation system ultrasonic sonicator probe with flow cell

20kHz 1500w Ultrasonic circulation system ultrasonic sonicator probe with flow cell

Optimize ultrasonic extraction of protein from Agaricus bisporus waste stipe:

To promote the utilization of Agaricus bisporus waste stipe and increase its economic value, protein were extracted from Agaricus bisporus waste stipe by ultrasonic extraction. The effect of ultrasonic power, ratio of liquid to solid, ultrasonic time, pH value on the yield of protein were investigated by single factor experiments, and optimized the parameters by orthogonal experiment use ultrasonic power, ratio of liquid to solid and pH value as factors. The results showed that as the ultrasonic time was 1 min, the parameters optimized by orthogonal experiment analysis were as follows: ultrasonic power 150 W, liquid-solid ratio 35:1 mL/g, pH value 10.0. Under these optimum conditions, the yield of protein extract from Agaricus bisporus waste stipe can be up to 6.87 g/100 g, which was 3.5 times higher than traditional alkali extraction, and shorten 60 times than traditional alkali extraction in time. As a simple, effective, fast, safe and no adverse reaction, ultrasonic extraction is effective extraction method, and can be used to extract all functional components.

 An ultrasonic cell disruptor (or ultrasonic homogenizer) is a device used in biological, chemical, and industrial laboratories to break open cells and disrupt their structure using high-frequency sound waves. The process involves creating ultrasonic vibrations that induce cavitation in liquids, leading to mechanical shearing forces that break cells apart and release their contents.

Parameter

Applications

1. Biology and Biochemistry:

• Cell lysis for DNA, RNA, or protein extraction.

• Homogenizing tissue samples.

2. Pharmaceuticals:

• Preparing emulsions and dispersions.

• Particle size reduction.

3. Nanotechnology:

• Dispersing nanoparticles in suspensions.

• Breaking down aggregates.

4. Chemical Synthesis:

• Enhancing reactions that benefit from high-energy environments.

5. Food and Beverage:

• Emulsification and mixing.

Working Principle

1. Ultrasonic Generator:

• Converts electrical energy into high-frequency electrical signals.

2. Transducer:

• Converts electrical signals into mechanical vibrations, generating ultrasonic waves.

3. Ultrasonic Probe (Horn):

• Transmits ultrasonic waves into the material, creating cavitation bubbles that implode, producing intense localized energy for processing.

4. Circulation System:

• A pump circulates the material through the ultrasonic treatment zone, ensuring consistent and thorough processing.

Advantages

1. High Efficiency:

• Rapid and effective processing due to intense cavitation effects.

2. Uniform Results:

• Continuous circulation ensures consistent treatment of all material.

3. Non-Thermal Processing:

• Ideal for heat-sensitive materials, as it avoids thermal degradation.

4. Scalability:

• Suitable for both lab-scale R&D and industrial-scale production.

5. Flexibility:

• Adjustable parameters allow customization for different materials and processes.